We found that morphine-activated bv-2 cells, including the upregulation of p38 mitogen-activated protein kinase p38 mapk phosphorylation, pro-inflammatory cytokines, and toll-like receptor-4 tlr-4 mrna expression, which was inhibited by metformin.
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tumor cells lacking functional mitochondria maintain lipid biosynthesis in the presence of metformin via glutamine-dependent reductive carboxylation, and display reduced sensitivity to metformin-induced proliferative arrest.